Sender, R., Fuchs, S. & Milo, R. Revised estimates for the variety of human and micro organism cells within the physique. PLoS Biol. 14, e1002533 (2016).
Odendall, C. & Kagan, J. C. Host-encoded sensors of micro organism: our home windows into the microbial world. Microbiol. Spectr. 7, 0011-2019 https://doi.org/10.1128/microbiolspec.BAI-0011-2019 (2019).
Sohlenkamp, C. & Geiger, O. Bacterial membrane lipids: variety in constructions and pathways. FEMS Microbiol. Rev. 40, 133–159 (2016).
Urdaneta, V. & Casadesús, J. Interactions between micro organism and bile salts within the gastrointestinal and hepatobiliary tracts. Entrance. Med. 4, 163 (2017).
Epand, R. M. & Epand, R. F. Lipid domains in bacterial membranes and the motion of antimicrobial brokers. Biochim. Biophys. Acta 1788, 289–294 (2009).
Nizet, V. Understanding how main bacterial pathogens subvert innate immunity to disclose novel therapeutic targets. J. Allergy Clin. Immunol. 120, 13–22 (2007).
Silhavy, T. J., Kahne, D. & Walker, S. The bacterial cell envelope. Chilly Spring Harb. Perspect. Biol. 2, a000414 (2010).
Strahl, H. & Errington, J. Bacterial membranes: construction, domains, and performance. Annu. Rev. Microbiol. 71, 519–538 (2017).
Schwechheimer, C. & Kuehn, M. J. Outer-membrane vesicles from Gram-negative micro organism: biogenesis and features. Nat. Rev. Microbiol. 13, 605–619 (2015).
Linares-Otoya, L. et al. Discovery of a widespread chemical signalling pathway within the Bacteroidota. Nature 646, 423–432 (2025).
Ryan, R. P. & Dow, J. M. Diffusible indicators and interspecies communication in micro organism. Microbiology 154, 1845–1858 (2008).
Motta, J.-P., Wallace, J. L., Buret, A. G., Deraison, C. & Vergnolle, N. Gastrointestinal biofilms in well being and illness. Nat. Rev. Gastroenterol. Hepatol. 18, 314–334 (2021).
Liu, H. Y., Prentice, E. L. & Webber, M. A. Mechanisms of antimicrobial resistance in biofilms. npj Antimicrob. Resist. 2, 27 (2024).
Flemming, H.-C. & Wingender, J. The biofilm matrix. Nat. Rev. Microbiol. 8, 623–633 (2010).
Wilson, M. M. & Bernstein, H. D. Floor-exposed lipoproteins: an rising secretion phenomenon in Gram-negative micro organism. Traits Microbiol. 24, 198–208 (2016).
Johnson, E. L., Heaver, S. L., Walters, W. A. & Ley, R. E. Microbiome and metabolic illness: revisiting the bacterial phylum Bacteroidetes. J. Mol. Med. 95, 1–8 (2017).
Slavetinsky, C., Kuhn, S. & Peschel, A. Bacterial aminoacyl phospholipids – biosynthesis and position in primary mobile processes and pathogenicity. Biochim. Biophys. Acta 1862, 1310–1318 (2017).
Rahlwes, Ok. C., Sparks, I. L. & Morita, Y. S. Cell partitions and membranes of actinobacteria. Subcell. Biochem. 92, 417–469 (2019).
Klag, Ok. et al. Dietary fats disrupts a commensal-host lipid community that promotes metabolic well being. Cell Metab. 38, 157–173.e9 (2026).
Hölzl, G. & Dörmann, P. Construction and performance of glycoglycerolipids in vegetation and micro organism. Prog. Lipid Res. 46, 225–243 (2007).
Raetz, C. R. H., Reynolds, C. M., Trent, M. S. & Bishop, R. E. Lipid A modification techniques in Gram-negative micro organism. Annu. Rev. Biochem. 76, 295–329 (2007).
Geiger, O., Padilla-Gómez, J. & López-Lara, I. M. in Biogenesis of Fatty Acids, Lipids and Membranes (ed. Geiger, O.) 123–137 https://doi.org/10.1007/978-3-319-50430-8_12 (Springer, 2019).
Godchaux, W. & Leadbetter, E. R. Sulfonolipids are localized within the outer membrane of the gliding bacterium Cytophaga johnsonae. Arch. Microbiol. 150, 42–47 (1988).
Sohlenkamp, C. in Biogenesis of Fatty Acids, Lipids and Membranes (ed. Geiger, O.) 109–122 https://doi.org/10.1007/978-3-319-50430-8_13 (Springer, 2019).
Jackson, M. The mycobacterial cell envelope-lipids. Chilly Spring Harb. Perspect. Med. 4, a021105 (2014).
Heaver, S. L., Johnson, E. L. & Ley, R. E. Sphingolipids in host-microbial interactions. Curr. Opin. Microbiol. 43, 92–99 (2018).
Kishino, S. et al. Polyunsaturated fatty acid saturation by intestine lactic acid micro organism affecting host lipid composition. Proc. Natl Acad. Sci. USA 110, 17808–17813 (2013).
Yasuda, S. et al. Elucidation of intestine microbiota-associated lipids utilizing LC-MS/MS and 16S rRNA sequence analyses. iScience 23, 101841 (2020).
Wieland Brown, L. C. et al. Manufacturing of α-galactosylceramide by a distinguished member of the human intestine microbiota. PLoS Biol. 11, e1001610 (2013).
Okino, N. et al. Two bacterial glycosphingolipid synthases answerable for the synthesis of glucuronosylceramide and α-galactosylceramide. J. Biol. Chem. 295, 10709–10725 (2020).
Brown, E. M., Clardy, J. & Xavier, R. J. Intestine microbiome lipid metabolism and its affect on host physiology. Cell Host Microbe 31, 173–186 (2023).
Ryan, E., Joyce, S. A. & Clarke, D. J. Membrane lipids from intestine microbiome-associated micro organism as structural and signalling molecules. Microbiology 169, 001315 (2023).
Morozumi, S., Ueda, M., Okahashi, N. & Arita, M. Buildings and features of the intestine microbial lipidome. Biochim. Biophys. Acta 1867, 159110 (2022).
Korkmaz, F. T. Trimming the fats: a short evaluation of lipids on the host-pathogen interface. Infect. Immun. 93, e0050624 (2025).
Strachan, D. P. Household measurement, an infection and atopy: the primary decade of the ‘hygiene speculation’. Thorax 55 (Suppl. 1), S2–S10 (2000).
Rook, G. A. W. The previous mates speculation: evolution, immunoregulation and important microbial inputs. Entrance. Allergy 4, 1220481 (2023).
Belkaid, Y. & Harrison, O. J. Homeostatic immunity and the microbiota. Immunity 46, 562–576 (2017).
Husebye, H. et al. Endocytic pathways regulate Toll-like receptor 4 signaling and hyperlink innate and adaptive immunity. EMBO J. 25, 683–692 (2006).
Park, B. S. et al. The structural foundation of lipopolysaccharide recognition by the TLR4-MD-2 advanced. Nature 458, 1191–1195 (2009).
Kagan, J. C. & Medzhitov, R. Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling. Cell 125, 943–955 (2006).
Kagan, J. C. et al. TRAM {couples} endocytosis of Toll-like receptor 4 to the induction of interferon-β. Nat. Immunol. 9, 361–368 (2008).
Raetz, C. R. H. & Whitfield, C. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 71, 635–700 (2002).
d’Hennezel, E., Abubucker, S., Murphy, L. O. & Cullen, T. W. Complete lipopolysaccharide from the human intestine microbiome silences Toll-like receptor signaling. mSystems 2, e00046-17, (2017).
Jacobson, A. N., Choudhury, B. P. & Fischbach, M. A. The biosynthesis of lipooligosaccharide from Bacteroides thetaiotaomicron. mBio 9, e02289-17 (2018).
Lindberg, A. A., Weintraub, A., Zähringer, U. & Rietschel, E. T. Construction-activity relationships in lipopolysaccharides of Bacteroides fragilis. Rev. Infect. Dis. 12 (Suppl. 2), S133–S141 (1990).
Cho, H.-S. et al. Construction of intestine microbial glycolipid modulates host inflammatory response. Cell 188, 5295–5312.e18 (2025).
Steimle, A. et al. Weak agonistic LPS restores intestinal immune homeostasis. Mol. Ther. 27, 1974–1991 (2019).
Stefan, Ok. L., Kim, M. V., Iwasaki, A. & Kasper, D. L. Commensal microbiota modulation of pure resistance to virus an infection. Cell 183, 1312–1324.e10 (2020).
Lai, H.-C. et al. Intestine microbiota modulates COPD pathogenesis: position of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide. Intestine 71, 309–321 (2022).
Lin, T.-L. et al. Like cures like: pharmacological exercise of anti-inflammatory lipopolysaccharides from intestine microbiome. Entrance. Pharmacol. 11, 554 (2020).
Vatanen, T. et al. Variation in microbiome LPS immunogenicity contributes to autoimmunity in people. Cell 165, 842–853 (2016).
Korneev, Ok. V. et al. Hypoacylated LPS from foodborne pathogen Campylobacter jejuni induces average TLR4-mediated inflammatory response in murine macrophages. Entrance. Cell. Infect. Microbiol. 8, 58 (2018).
Vinogradov, E., Perry, M. B. & Conlan, J. W. Structural evaluation of Francisella tularensis lipopolysaccharide. Eur. J. Biochem. 269, 6112–6118 (2002).
John, C. M. et al. Lipooligosaccharide constructions of invasive and provider isolates of Neisseria meningitidis are correlated with pathogenicity and carriage. J. Biol. Chem. 291, 3224–3238 (2016).
Kawahara, Ok., Tsukano, H., Watanabe, H., Lindner, B. & Matsuura, M. Modification of the construction and exercise of lipid A in Yersinia pestis lipopolysaccharide by development temperature. Infect. Immun. 70, 4092–4098 (2002).
Preston, A., Mandrell, R. E., Gibson, B. W. & Apicella, M. A. The lipooligosaccharides of pathogenic Gram-negative micro organism. Crit. Rev. Microbiol. 22, 139–180 (1996).
Zarantonelli, M. L., Huerre, M., Taha, M.-Ok. & Alonso, J.-M. Differential position of lipooligosaccharide of Neisseria meningitidis in virulence and inflammatory response throughout respiratory an infection in mice. Infect. Immun. 74, 5506–5512 (2006).
Ram, S. et al. A novel sialylation website on Neisseria gonorrhoeae lipooligosaccharide hyperlinks heptose II lactose expression with pathogenicity. Infect. Immun. 86, e00285-18 (2018).
Needham, B. D. & Trent, M. S. Fortifying the barrier: the affect of lipid A remodelling on bacterial pathogenesis. Nat. Rev. Microbiol. 11, 467–481 (2013).
Mathew, B., Aoyagi, Ok. L. & Fisher, M. A. Yersinia pestis lipopolysaccharide transforming confers resistance to a Xenopsylla cheopis cecropin. ACS Infect. Dis. 7, 2536–2545 (2021).
Pizzuto, M. et al. Ornithine lipid is a partial TLR4 agonist and NLRP3 activator. Cell Rep. 43, 114788 (2024).
Zhang, Q. et al. Genetic mapping of microbial and host traits reveals manufacturing of immunomodulatory lipids by Akkermansia muciniphila within the murine intestine. Nat. Microbiol. 8, 424–440 (2023).
Córdoba-Castro, L. A. et al. Ornithine lipids in Burkholderia spp. pathogenicity. Entrance. Mol. Biosci. 7, 610932 (2020).
Older, E. A. et al. Biosynthetic enzyme evaluation identifies a protecting position for TLR4-acting intestine microbial sulfonolipids in inflammatory bowel illness. Nat. Commun. 15, 9371 (2024).
Walker, A. et al. Sulfonolipids as novel metabolite markers of Alistipes and Odoribacter affected by high-fat diets. Sci. Rep. 7, 11047 (2017).
Claypool, S. M. & Koehler, C. M. The complexity of cardiolipin in well being and illness. Traits Biochem. Sci. 37, 32–41 (2012).
Pizzuto, M. et al. Saturation of acyl chains converts cardiolipin from an antagonist to an activator of Toll-like receptor-4. Cell. Mol. Life Sci. 76, 3667–3678 (2019).
Wong, S. W. et al. Fatty acids modulate Toll-like receptor 4 activation via regulation of receptor dimerization and recruitment into lipid rafts in a reactive oxygen species-dependent method. J. Biol. Chem. 284, 27384–27392 (2009).
Nguyen, M.-T., Matsuo, M., Niemann, S., Herrmann, M. & Götz, F. Lipoproteins in Gram-positive micro organism: abundance, perform, health. Entrance. Microbiol. 11, 582582 (2020).
Takeuchi, O. et al. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int. Immunol. 13, 933–940 (2001).
Jin, M. S. et al. Crystal construction of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide. Cell 130, 1071–1082 (2007).
O’Neill, L. A. J., Golenbock, D. & Bowie, A. G. The historical past of Toll-like receptors — redefining innate immunity. Nat. Rev. Immunol. 13, 453–460 (2013).
Oosting, M. et al. Human TLR10 is an anti-inflammatory pattern-recognition receptor. Proc. Natl Acad. Sci. USA 111, E4478–E4484 (2014).
Rolf, N., Kariminia, A., Ivison, S., Reid, G. S. & Schultz, Ok. R. Heterodimer-specific TLR2 stimulation leads to divergent practical outcomes in B-cell precursor acute lymphoblastic leukemia: immunomodulation. Eur. J. Immunol. 45, 1980–1990 (2015).
Monlish, D. A. et al. TLR2/6 signaling promotes the enlargement of premalignant hematopoietic stem and progenitor cells within the NUP98-HOXD13 mouse mannequin of MDS. Exp. Hematol. 88, 42–55 (2020).
Nguyen, M.-T. et al. Lipid moieties on lipoproteins of commensal and non-commensal staphylococci induce differential immune responses. Nat. Commun. 8, 2246 (2017).
Gardiner, J. H. 4th et al. Lipoprotein N-acylation in Staphylococcus aureus is catalyzed by a two-component acyl transferase system. mBio 11, e01619-20 (2020).
Machata, S. et al. Lipoproteins of listeria monocytogenes are crucial for virulence and TLR2-mediated immune activation. J. Immunol. 181, 2028–2035 (2008).
Armbruster, Ok. M., Komazin, G. & Meredith, T. C. Copper-induced expression of a transmissible lipoprotein intramolecular transacylase alters lipoprotein acylation and the Toll-like receptor 2 response to Listeria monocytogenes. J. Bacteriol. 201, e00195-19 (2019).
Hirschfeld, M. et al. Leading edge: inflammatory signaling by Borrelia burgdorferi lipoproteins is mediated by toll-like receptor 2. J. Immunol. 163, 2382–2386 (1999).
Wooten, R. M. et al. Toll-like receptor 2 is required for innate, however not acquired, host protection to Borrelia burgdorferi. J. Immunol. 168, 348–355 (2002).
Chen, X. et al. Branched chain fatty acid synthesis drives tissue-specific innate immune response and an infection dynamics of Staphylococcus aureus. PLoS Pathog. 17, e1009930 (2021).
Nguyen, M. T., Hanzelmann, D., Härtner, T., Peschel, A. & Götz, F. Pores and skin-specific unsaturated fatty acids enhance the Staphylococcus aureus innate immune response. Infect. Immun. 84, 205–215 (2015).
Chen, X. & Alonzo, F. third. Bacterial lipolysis of immune-activating ligands promotes evasion of innate defenses. Proc. Natl Acad. Sci. USA 116, 3764–3773 (2019).
Grayczyk, J. P., Harvey, C. J., Laczkovich, I. & Alonzo, F. third A lipoylated metabolic protein launched by Staphylococcus aureus suppresses macrophage activation. Cell Host Microbe 22, 678–687.e9 (2017).
Hu, W. & Spaink, H. P. The position of TLR2 in infectious ailments attributable to mycobacteria: from cell biology to therapeutic goal. Biology 11, 246 (2022).
Shukla, S., Richardson, E. T., Drage, M. G., Growth, W. H. & Harding, C. V. Mycobacterium tuberculosis lipoprotein and lipoglycan binding to Toll-like receptor 2 correlates with agonist exercise and practical outcomes. Infect. Immun. 86, e00450-18 (2018).
Gilleron, M., Nigou, J., Nicolle, D., Quesniaux, V. & Puzo, G. The acylation state of mycobacterial lipomannans modulates innate immunity response via Toll-like receptor 2. Chem. Biol. 13, 39–47 (2006).
Drennan, M. B. et al. Toll-like receptor 2-deficient mice succumb to Mycobacterium tuberculosis an infection. Am. J. Pathol. 164, 49–57 (2004).
Gehring, A. J., Dobos, Ok. M., Belisle, J. T., Harding, C. V. & Growth, W. H. Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J. Immunol. 173, 2660–2668 (2004).
Cario, E., Gerken, G. & Podolsky, D. Ok. Toll-like receptor 2 controls mucosal irritation by regulating epithelial barrier perform. Gastroenterology 132, 1359–1374 (2007).
Pierik, M. et al. Toll-like receptor-1, -2, and -6 polymorphisms affect illness extension in inflammatory bowel ailments. Inflamm. Bowel Dis. 12, 1–8 (2006).
Financial institution, S. et al. Polymorphisms within the inflammatory pathway genes TLR2, TLR4, TLR9, LY96, NFKBIA, NFKB1, TNFA, TNFRSF1A, IL6R, IL10, IL23R, PTPN22, and PPARG are related to susceptibility of inflammatory bowel illness in a Danish cohort. PLoS ONE 9, e98815 (2014).
Dempsey, E. & Corr, S. C. Lactobacillus spp. for gastrointestinal well being: present and future views. Entrance. Immunol. 13, 840245 (2022).
Lee, I.-C. et al. Lipoproteins contribute to the anti-inflammatory capability of Lactobacillus plantarum WCFS1. Entrance. Microbiol. 11, 1822 (2020).
Cani, P. D., Depommier, C., Derrien, M., Everard, A. & de Vos, W. M. Akkermansia muciniphila: paradigm for next-generation useful microorganisms. Nat. Rev. Gastroenterol. Hepatol. 19, 625–637 (2022).
Bae, M. et al. Akkermansia muciniphila phospholipid induces homeostatic immune responses. Nature 608, 168–173 (2022).
Shah, H. N. & Collins, M. D. Genus Bacteroides. A chemotaxonomical perspective. J. Appl. Bacteriol. 55, 403–416 (1983).
Plovier, H. et al. A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in overweight and diabetic mice. Nat. Med. 23, 107–113 (2017).
Ottman, N. et al. Pili-like proteins of Akkermansia muciniphila modulate host immune responses and intestine barrier perform. PLoS ONE 12, e0173004 (2017).
Wang, L. et al. A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis related tumourigenesis by modulation of CD8+ T cells in mice. Intestine 69, 1988–1997 (2020).
Garcia-Vello, P. et al. The lipooligosaccharide of the intestine symbiont Akkermansia muciniphila displays a outstanding construction and TLR signaling capability. Nat. Commun. 15, 8411 (2024).
Chavarría-Velázquez, C. O., Torres-Martínez, A. C., Montaño, L. F. & Rendón-Huerta, E. P. TLR2 activation induced by H. pylori LPS promotes the differential expression of claudin-4, -6, -7 and -9 by way of both STAT3 and ERK1/2 in AGS cells. Immunobiology 223, 38–48 (2018).
Di Lorenzo, F. et al. Pairing Bacteroides vulgatus LPS construction with its immunomodulatory results on human mobile fashions. ACS Cent. Sci. 6, 1602–1616 (2020).
Pither, M. D. et al. Bacteroides thetaiotaomicron rough-type lipopolysaccharide: the chemical construction and the immunological exercise. Carbohydr. Polym. 297, 120040 (2022).
Clark, R. B. et al. Serine lipids of Porphyromonas gingivalis are human and mouse Toll-like receptor 2 ligands. Infect. Immun. 81, 3479–3489 (2013).
Holzheimer, M. et al. Uneven complete synthesis of mycobacterial diacyl trehaloses demonstrates a job for lipid construction in immunogenicity. ACS Chem. Biol. 15, 1835–1841 (2020).
Lin, Z. et al. Uneven complete synthesis and structural revision of DAT2, an antigenic glycolipid from Mycobacterium tuberculosis. Angew. Chem. Int. Ed. 136, e202318582 (2024).
Huang, S. et al. CD1 lipidomes reveal lipid-binding motifs and size-based antigen-display mechanisms. Cell 186, 4583–4596.e13 (2023).
Lynch, A., Tammireddy, S. R., Doherty, M. Ok., Whitfield, P. D. & Clarke, D. J. The glycine lipids of Bacteroides thetaiotaomicron are essential for health throughout development in vivo and in vitro. Appl. Environ. Microbiol. 85, e02157-18 (2019).
Cohen, L. J. et al. Useful metagenomic discovery of bacterial effectors within the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist. Proc. Natl Acad. Sci. USA 112, E4825–E4834 (2015).
Nichols, F. C. et al. A novel phosphoglycerol serine-glycine lipodipeptide of Porphyromonas gingivalis is a TLR2 ligand. J. Lipid Res. 61, 1645–1657 (2020).
Mirretta Barone, C. et al. Spatially resolved lipidomics exhibits conditional switch of lipids produced by Bacteroides thetaiotaomicron into the mouse intestine. Cell Host Microbe 32, 1025–1036.e5 (2024).
Lin, J. et al. Porphyromonas gingivalis exacerbates ligature-induced, RANKL-dependent alveolar bone resorption by way of differential regulation of Toll-like receptor 2 (TLR2) and TLR4. Infect. Immun. 82, 4127–4134 (2014).
Nichols, F. C. et al. Glycine lipids of Porphyromonas gingivalis are agonists for Toll-like receptor 2. Infect. Immun. 88, e00877-19 (2020).
Papadopoulos, G. et al. Macrophage-specific TLR2 signaling mediates pathogen-induced TNF-dependent inflammatory oral bone loss. J. Immunol. 190, 1148–1157 (2013).
Millar, C. L. et al. Intestine microbiome-derived glycine lipids are diet-dependent modulators of hepatic harm and atherosclerosis. J. Lipid Res. 63, 100192 (2022).
Barral, D. C. & Brenner, M. B. CD1 antigen presentation: the way it works. Nat. Rev. Immunol. 7, 929–941 (2007).
James, C. A. et al. CD1b tetramers determine T cells that acknowledge pure and artificial diacylated sulfoglycolipids from Mycobacterium tuberculosis. Cell Chem. Biol. 25, 392–402.e14 (2018).
Moody, D. B. et al. CD1b-mediated T cell recognition of a glycolipid antigen generated from mycobacterial lipid and host carbohydrate throughout an infection. J. Exp. Med. 192, 965–976 (2000).
Sieling, P. A. et al. CD1-restricted T cell recognition of microbial lipoglycan antigens. Science 269, 227–230 (1995).
Moody, D. B. et al. T cell activation by lipopeptide antigens. Science 303, 527–531 (2004).
Ly, D. et al. CD1c tetramers detect ex vivo T cell responses to processed phosphomycoketide antigens. J. Exp. Med. 210, 729–741 (2013).
Moody, D. B. et al. CD1c-mediated T-cell recognition of isoprenoid glycolipids in Mycobacterium tuberculosis an infection. Nature 404, 884–888 (2000).
Goldberg, M. F., Saini, N. Ok. & Porcelli, S. A. Evasion of innate and adaptive immunity by Mycobacterium tuberculosis. Microbiol. Spectr. 2, 0005-2013 https://doi.org/10.1128/microbiolspec.MGM2-0005-2013 (2014).
Ben-Menachem, G., Kubler-Kielb, J., Coxon, B., Yergey, A. & Schneerson, R. A newly found cholesteryl galactoside from Borrelia burgdorferi. Proc. Natl Acad. Sci. USA 100, 7913–7918 (2003).
Reinink, P. et al. CD1b presents self and Borrelia burgdorferi diacylglycerols to human T cells. Eur. J. Immunol. 49, 737–746 (2019).
Mansour, S. et al. Cholesteryl esters stabilize human CD1c conformations for recognition by self-reactive T cells. Proc. Natl Acad. Sci. USA 113, E1266–E1275 (2016).
Singh, A. Ok., Tripathi, P. & Cardell, S. L. Kind II NKT cells: an elusive inhabitants with immunoregulatory properties. Entrance. Immunol. 9, 1969 (2018).
Tatituri, R. V. V. et al. Recognition of microbial and mammalian phospholipid antigens by NKT cells with numerous TCRs. Proc. Natl Acad. Sci. USA 110, 1827–1832 (2013).
Wolf, B. J. et al. Identification of a potent microbial lipid antigen for numerous NKT cells. J. Immunol. 195, 2540–2551 (2015).
Kawano, T. et al. CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides. Science 278, 1626–1629 (1997).
Kinjo, Y. et al. Recognition of bacterial glycosphingolipids by pure killer T cells. Nature 434, 520–525 (2005).
Kinjo, Y. et al. Pure Sphingomonas glycolipids fluctuate tremendously of their capacity to activate pure killer T cells. Chem. Biol. 15, 654–664 (2008).
Kinjo, Y. et al. Pure killer T cells acknowledge diacylglycerol antigens from pathogenic micro organism. Nat. Immunol. 7, 978–986 (2006).
Ito, Y. et al. Helicobacter pylori cholesteryl α-glucosides contribute to its pathogenicity and immune response by pure killer T cells. PLoS ONE 8, e78191 (2013).
Kinjo, Y. et al. Invariant pure killer T cells acknowledge glycolipids from pathogenic Gram-positive micro organism. Nat. Immunol. 12, 966–974 (2011).
Olszak, T. et al. Microbial publicity throughout formative years has persistent results on pure killer T cell perform. Science 336, 489–493 (2012).
An, D. et al. Sphingolipids from a symbiotic microbe regulate homeostasis of host intestinal pure killer T cells. Cell 156, 123–133 (2014).
Oh, S. F. et al. Host immunomodulatory lipids created by symbionts from dietary amino acids. Nature 600, 302–307 (2021).
Yoo, J.-S. et al. Human intestine micro organism produce structurally associated monoglycolipids with contrasting immune features. Nat. Microbiol. 10, 2797–2807 (2025).
Muraille, E. & Leo, O. Revisiting the Th1/Th2 paradigm. Scand. J. Immunol. 47, 1–9 (1998).
Rogozynski, N. P. & Dixon, B. The Th1/Th2 paradigm: a misrepresentation of helper T cell plasticity. Immunol. Lett. 268, 106870 (2024).
Romanò, C. & Clausen, M. H. Chemical biology of αGalCer: a chemist’s toolbox for the stimulation of invariant pure killer T (iNKT) cells. Eur. J. Org. Chem. 2022, e202200246 (2022).
Kim, S., Lalani, S., Parekh, V. V., Wu, L. & Van Kaer, L. Glycolipid ligands of invariant pure killer T cells as vaccine adjuvants. Professional Rev. Vaccines 7, 1519–1532 (2008).
Van Kaer, L. & Wu, L. Therapeutic potential of invariant pure killer T cells in autoimmunity. Entrance. Immunol. 9, 519 (2018).
Lee, J., Son, S., Lee, M. & Park, S. B. Improvement of potential immunomodulatory ligands focusing on pure killer T cells impressed by intestine symbiont-derived glycolipids. Commun. Chem. 8, 98 (2025).
Facciotti, F. et al. Peroxisome-derived lipids are self antigens that stimulate invariant pure killer T cells within the thymus. Nat. Immunol. 13, 474–480 (2012).
Gumperz, J. E. et al. Murine CD1d-restricted T cell recognition of mobile lipids. Immunity 12, 211–221 (2000).
Heaver, S. L. et al. Characterization of inositol lipid metabolism in gut-associated Bacteroidetes. Nat. Microbiol. 7, 986–1000 (2022).
Shahine, A. et al. A molecular foundation of human T cell receptor autoreactivity towards self-phospholipids. Sci. Immunol. 2, eaao1384 (2017).
Mansouri, A., Akthar, I. & Miyamoto, A. TLR2 and TLR4 bridge physiological and pathological irritation within the reproductive system. Commun. Biol. 8, 1008 (2025).
Spitzer, J. H., Visintin, A., Mazzoni, A., Kennedy, M. N. & Segal, D. M. Toll-like receptor 1 inhibits Toll-like receptor 4 signaling in endothelial cells. Eur. J. Immunol. 32, 1182–1187 (2002).
Nagarajan, N. A. & Kronenberg, M. Invariant NKT cells amplify the innate immune response to lipopolysaccharide. J. Immunol. 178, 2706–2713 (2007).
Poad, B. L. J. et al. Revolutions in lipid isomer decision: utility of ultrahigh-resolution ion mobility to disclose lipid variety. Anal. Chem. 95, 15917–15923 (2023).
Xia, T. et al. Deep-profiling of phospholipidome by way of fast orthogonal separations and isomer-resolved mass spectrometry. Nat. Commun. 14, 4263 (2023).
Liu, Y., Xia, Y. & Zhang, W. Structural lipidomics enabled by isomer-resolved tandem mass spectrometry. Anal. Chem. 97, 4275–4286 (2025).
Mayfield, J. A. et al. Mycobacteria that trigger tuberculosis have retained ancestrally acquired genes for the biosynthesis of chemically numerous terpene nucleosides. PLoS Biol. 22, e3002813 (2024).
Good, C. J. et al. Uncovering lipid dynamics in Staphylococcus aureus osteomyelitis utilizing multimodal imaging mass spectrometry. Cell Chem. Biol. 31, 1852–1868.e5 (2024).
Lee, M.-T., Le, H. H. & Johnson, E. L. Dietary sphinganine is selectively assimilated by members of the mammalian intestine microbiome. J. Lipid Res. 62, 100034 (2021).
Luan, L., Frederick, N. P. & Baskin, J. M. Rising approaches for learning lipid dynamics, metabolism, and interactions in cells. Annu. Rev. Biochem. 94, 417–446 (2025).
Rodriguez-Rivera, F. P., Zhou, X., Theriot, J. A. & Bertozzi, C. R. Visualization of mycobacterial membrane dynamics in reside cells. J. Am. Chem. Soc. 139, 3488–3495 (2017).
Wuo, M. G. et al. Fluorogenic probes of the mycobacterial membrane as reporters of antibiotic motion. J. Am. Chem. Soc. 146, 17669–17678 (2024).
Brown, E. M. et al. Bacteroides sphingolipids promote anti-inflammatory responses via the mevalonate pathway. Cell Host Microbe 33, 901–914.e6 (2025).
Yang, T. et al. Focusing on symbionts by apolipoprotein L proteins modulates intestine immunity. Nature 643, 210–218 (2025).
Dohnalová, L. et al. A microbiome-dependent gut-brain pathway regulates motivation for train. Nature 612, 739–747 (2022).
Le, H. H., Lee, M.-T., Besler, Ok. R. & Johnson, E. L. Host hepatic metabolism is modulated by intestine microbiota-derived sphingolipids. Cell Host Microbe 30, 798–808.e7 (2022).
Takeuchi, T. et al. Fatty acid overproduction by intestine commensal microbiota exacerbates weight problems. Cell Metab. 35, 361–375.e9 (2023).
Chang, F.-Y. et al. Intestine-inhabiting clostridia construct human GPCR ligands by conjugating neurotransmitters with diet- and human-derived fatty acids. Nat. Microbiol. 6, 792–805 (2021).
Lynch, J. B. et al. Intestine microbiota Turicibacter strains differentially modify bile acids and host lipids. Nat. Commun. 14, 3669 (2023).
Liu, C. et al. Intestine commensal Christensenella minuta modulates host metabolism by way of acylated secondary bile acids. Nat. Microbiol. 9, 434–450 (2024).
Chen, Ok.-L., Kuo, T.-H. & Hsu, C.-C. Mapping lipid C═C isomer profiles of human intestine micro organism via a novel structural lipidomics workflow assisted by chemical epoxidation. Anal. Chem. 96, 17526–17536 (2024).
Wooden, P. L., Le, A. & Palazzolo, D. L. Comparative lipidomics of oral commensal and opportunistic micro organism. Metabolites 14, 240 (2024).
Kleiboeker, B. A., Frankfater, C., Davey, M. E. & Hsu, F.-F. Lipidomic evaluation of Porphyromonas gingivalis reveals novel glycerol bisphosphoceramide, phosphatidyl-, and phosphoglycerol dipeptide lipid households. J. Lipid Res. 64, 100470 (2023).
Frankfater, C. F., Sartorio, M. G., Valguarnera, E., Feldman, M. F. & Hsu, F.-F. Lipidome of the Bacteroides genus containing new peptidolipid and sphingolipid households revealed by multiple-stage mass spectrometry. Biochemistry 62, 1160–1180 (2023).
Ryan, E., Gonzalez Pastor, B., Gethings, L. A., Clarke, D. J. & Joyce, S. A. Lipidomic evaluation reveals variations in Bacteroides species pushed largely by plasmalogens, glycerophosphoinositols and sure sphingolipids. Metabolites 13, 360 (2023).
Xiao, X. et al. Prevotella copri variants amongst a single host diverge in sphingolipid manufacturing. mBio 15, e0240923 (2024).
Cheng, J., Venkatesh, S., Ke, Ok., Barratt, M. J. & Gordon, J. I. A human intestine Faecalibacterium prausnitzii fatty acid amide hydrolase. Science 386, eado6828 (2024).
Wu, X. et al. Identification of 3-ketocapnine reductase exercise inside the human microbiota. mLife 3, 307–316 (2024).
Radka, C. D., Miller, D. J., Frank, M. W. & Rock, C. O. Biochemical characterization of step one in sulfonolipid biosynthesis in Alistipes finegoldii. J. Biol. Chem. 298, 102195 (2022).
Lee, M.-T., Le, H. H., Besler, Ok. R. & Johnson, E. L. Identification and characterization of 3-ketosphinganine reductase exercise encoded on the BT_0972 locus in Bacteroides thetaiotaomicron. J. Lipid Res. 63, 100236 (2022).
Esposti, M. D. Eukaryotes inherited inositol lipids from micro organism: implications for the fashions of eukaryogenesis. FEBS Lett. 597, 2484–2496 (2023).
Stankeviciute, G. et al. Convergent evolution of bacterial ceramide synthesis. Nat. Chem. Biol. 18, 305–312 (2022).
Wang, X., Ribeiro, A. A., Guan, Z., Abraham, S. N. & Raetz, C. R. H. Attenuated virulence of a Francisella mutant missing the lipid A 4’-phosphatase. Proc. Natl Acad. Sci. USA 104, 4136–4141 (2007).
Kong, Q. et al. Phosphate teams of lipid A are important for Salmonella enterica serovar Typhimurium virulence and have an effect on innate and adaptive immunity. Infect. Immun. 80, 3215–3224 (2012).
Mata-Haro, V. et al. The vaccine adjuvant monophosphoryl lipid A as a TRIF-biased agonist of TLR4. Science 316, 1628–1632 (2007).
Coats, S. R. et al. Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1- and 4’-phosphatase actions. Cell. Microbiol. 11, 1587–1599 (2009).
Kurokawa, Ok. et al. Novel bacterial lipoprotein constructions conserved in low-GC content material Gram-positive micro organism are acknowledged by Toll-like receptor 2. J. Biol. Chem. 287, 13170–13181 (2012).
Kang, J. Y. et al. Recognition of lipopeptide patterns by Toll-like receptor 2-Toll-like receptor 6 heterodimer. Immunity 31, 873–884 (2009).
Yao, J., Cherian, P. T., Frank, M. W. & Rock, C. O. Chlamydia trachomatis depends on autonomous phospholipid synthesis for membrane biogenesis. J. Biol. Chem. 290, 18874–18888 (2015).
Belin, B. J. et al. Hopanoid lipids: from membranes to plant–micro organism interactions. Nat. Rev. Microbiol. 16, 304–315 (2018).
Conover, G. M. et al. Phosphatidylcholine synthesis is required for optimum perform of Legionella pneumophila virulence determinants. Cell. Microbiol. 10, 514–528 (2008).
Welander, P. V. & Summons, R. E. Discovery, taxonomic distribution, and phenotypic characterization of a gene required for 3-methylhopanoid manufacturing. Proc. Natl Acad. Sci. USA 109, 12905–12910 (2012).
Lamb, D. C. et al. Lanosterol biosynthesis within the prokaryote Methylococcus capsulatus: perception into the evolution of sterol biosynthesis. Mol. Biol. Evol. 24, 1714–1721 (2007).
Olsen, R. W. & Ballou, C. E. Acyl phosphatidylglycerol. J. Biol. Chem. 246, 3305–3313 (1971).
Klein, S. et al. Adaptation of Pseudomonas aeruginosa to varied circumstances consists of tRNA-dependent formation of alanyl-phosphatidylglycerol. Mol. Microbiol. 71, 551–565 (2009).
Livermore, B. P. & Johnson, R. C. Lipids of the Spirochaetales: comparability of the lipids of a number of members of the genera Spirochaeta, Treponema, and Leptospira. J. Bacteriol. 120, 1268–1273 (1974).
Moribayashi, A., Goto, N., Arimitsu, Y., Himeno, Ok. & Tatsuki, S. Lipids and fatty acids of Leptospira interrogans serovar copenhageni virulent pressure Shibaura. Jpn. J. Med. Sci. Biol. 44, 87–97 (1991).
Kent, C., Gee, P., Lee, S. Y., Bian, X. & Fenno, J. C. A CDP-choline pathway for phosphatidylcholine biosynthesis in Treponema denticola. Mol. Microbiol. 51, 471–481 (2004).
Huang, X. et al. Excessive-fat eating regimen will increase circulating palmitic acid produced by intestine Bacteroides thetaiotaomicron to advertise thrombosis. Cell Rep. Med. 6, 102260 (2025).
Hagen, P. O. Lipids of Sphaerophorus ridiculosis: plasmalogen composition. J. Bacteriol. 119, 643–645 (1974).
Elwell, C. A. & Engel, J. N. Lipid acquisition by intracellular chlamydiae. Cell. Microbiol. 14, 1010–1018 (2012).
Percy, M. G. & Gründling, A. Lipoteichoic acid synthesis and performance in Gram-positive micro organism. Annu. Rev. Microbiol. 68, 81–100 (2014).
Schwan, W. R., Demuth, A., Kuhn, M. & Goebel, W. Phosphatidylinositol-specific phospholipase C from Listeria monocytogenes contributes to intracellular survival and development of Listeria innocua. Infect. Immun. 62, 4795–4803 (1994).
Goldfine, H. Plasmalogens in micro organism, sixty years on. Entrance. Mol. Biosci. 9, 962757 (2022).
Guan, Z. & Goldfine, H. Lipid variety in clostridia. Biochim. Biophys. Acta 1866, 158966 (2021).
Alvarez, H. M. Triacylglycerol and wax ester-accumulating equipment in prokaryotes. Biochimie 120, 28–39 (2016).